Regarding the suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.
Cell Culture in Bioreactors
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What factors should be considered when establishing bioreactor cell cultures?
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The shear force on the cell culture in bioreactors
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Impellers
- Blades that are axial, or have a 45°-degree angle, provide gentle yet effective mixing that is suitable for sensitive cell cultures. Examples are pitched-blade impellers and marine impellers.
- Radial blades are suitable for more robust cell cultures, hence are more commonly used for microbial fermentation. An example is the Rushton impeller.
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Gassing
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Regulation of critical process parameters
Critical process parameters
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Bioprocess monitoring and control
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Feeding bioreactor cell cultures
Bioreactor operation modes
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Perfusion
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Prevent contamination of cell cultures in bioreactors
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Sources of contamination
- The integration of equipment such as sensors
- Improperly installed or damaged seals
- Improper cleaning between uses
These bioreactors also have an increased risk of cross-contamination, if the equipment is used to culture different types of cells or the production of different types of proteins.
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Bioprocess scalability
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Frequently used cell types tailored towards your bioprocessing application of choice
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Chinese hamster ovary (CHO) cell culture in bioreactors
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Stem cell culture in bioreactors
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T cell culture in bioreactors
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HEK293 cell culture in bioreactors
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Vero cell culture in bioreactors
- Learn more about Vero cell culture using Fibra-Cel® Disks or using Cytodex® microcarriers in Eppendorf bioreactor systems.
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Sf9 cell culture in bioreactors
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